TY - JOUR AU - Riabovol, Vasyl AU - Kurchenko, Andrii AU - Yavorovskyi, Alexander AU - Savchenko, Viktoriia AU - Taran, Nina PY - 2021/11/28 Y2 - 2024/03/28 TI - A STUDY OF THE INFLUENCE OF PHOTOACTIVE TITANIUM COMPOSITE NANOPARTICLES ON THE FUNCTIONAL ACTIVITY OF CYTOKINE-PRODUCING MONONUCLEAR BLOOD CELLS IN VITRO JF - Immunology and Allergology: Science and Practice JA - Immun. and Allerg.: Sci. and Pract. VL - 0 IS - 3 SE - Articles DO - 10.37321/immunology.2021.3-03 UR - https://immunology.org.ua/index.php/journal/article/view/53 SP - 23-30 AB - Objective. To study the effect of photoactive nanoparticles on the functional activity of peripheral mononuclear blood cells (PMBC) of healthy donors for the in-vitro production of cytokines: IL-1, IL-4, IL-6, TNF-α.Materials and methods. Subjects used in the study are: crystalline titanium dioxide nanopowder – anatase with a nanoparticle size of 21-28 nm; nanocomposite of titanium dioxide with 4% nanosilver, nanoparticle size 17-22 nm; nanosized silver; developed by the Frantsevich Institute of Materials Science.Mononuclear cells of peripheral blood were cultured ahead of time by deriving them from peripheral blood of volunteer donors. The cell suspension was further incubated without a stimulating agent and with PHA stimulation using mitogen (30 μg/ml concentration), as well as with the addition of the studied nanoparticles (30 μg/ml concentration). Finally, the concentration of cytokines was determined by means of an immunosorbent assay.Results. Compared to spontaneous production, the stimulation of mononuclear cells with nanoparticles in the according concentration of nanoTiO2 resulted inthe production of the IL-1 increasing to 60.38±9.04 pkg/ml (1.79 times P<0.01), for nanoTiO2-Ag – 91.75±11.49 pkg/ml (2.73 times P<0.01), for nanoAg – 77.35±6.2 pkg/ml (2.3 times P<0.01).When nanoparticles were added to the mononuclear cells in the according concentration of nanoTiO2-Ag, the production of IL-6 increased to 75.8 (CI 71.8-87.4) pkg/ml (5.41 times P<0.01), nanoAg – 52,0 (CI 46.0-63.1) pkg/ml (3.71 times P<0.05), compared to spontaneous production.When mononuclear cells were stimulated with nanoparticles in the according concentration of nano-TiO2-Ag, the production of TNF-α increased to 80.3 (CI 68.0-82.4) pkg/ml (5.43 times P<0.01), nanoAg – 37,8 (CI 36.7-54.2) pkg/ml (2.55 times P<0.05), compared to spontaneous production.Compared to spontaneous production, the stimulation of mononuclear cells with nanoparticles in the according concentration of nanoTiO2-Ag resulted in theproduction of IL-4 increasing to 60.8 (CI 52.8-77.6) pkg/ml (3.45 times P<0.01), nanoAg - 41,7 (CI 34.7-49.1) pkg/ml (2.37 times P<0.01).Conclusion. The in vitro experiments have evidenced that nanoparticles at a concentration of 30 μg/ml for nanoTiO2-Ag and nanoAg are central in increasingfunctional activity of PMBC producing proinflammatory cytokines IL-1, IL-6, TNF-α and producing IL-4 in donors (P<0.05). The aforementioned data demonstrates a negative effect of nanoparticles on nanotechnology industry workers causing them to develop chronic inflammatory response and allergic reactions. ER -