A STUDY OF THE INFLUENCE OF PHOTOACTIVE TITANIUM COMPOSITE NANOPARTICLES ON THE FUNCTIONAL ACTIVITY OF CYTOKINE-PRODUCING MONONUCLEAR BLOOD CELLS IN VITRO

  • Vasyl Riabovol PhD student of the Department of Hygiene and Ecology №2 of the National Medical University named after O. Bogomolets
  • Andrii Kurchenko Doctor of Medical Sciences, Professor, Head of the Department of Clinical Immunology and Allergology with the Section of Medical Genetics of the National Medical University named after O. Bogomolets
  • Alexander Yavorovskyi Doctor of Medical Sciences, Professor, Academician of the National Academy of Medical Sciences of Ukraine, Head of the Department of Hygiene and Ecology №2 of the National Medical University named after O. Bogomolets
  • Viktoriia Savchenko Ph.D., senior researcher lab. of Immunology, Institute of Nephrology, National Academy of Medical Sciences of Ukraine
  • Nina Taran PhD, Associate Professor of Hygiene and Ecology №2 National Medical University named after O. Bogomolets
Keywords: nanoparticles, immunotoxicology, titanium dioxide nanoparticles, nanoparticles of composite titanium dioxide with silver, cytokines IL-1, IL-6, TNF-α, IL-4

Abstract

Objective. To study the effect of photoactive nanoparticles on the functional activity of peripheral mononuclear blood cells (PMBC) of healthy donors for the in-vitro production of cytokines: IL-1, IL-4, IL-6, TNF-α.
Materials and methods. Subjects used in the study are: crystalline titanium dioxide nanopowder – anatase with a nanoparticle size of 21-28 nm; nanocomposite of titanium dioxide with 4% nanosilver, nanoparticle size 17-22 nm; nanosized silver; developed by the Frantsevich Institute of Materials Science.
Mononuclear cells of peripheral blood were cultured ahead of time by deriving them from peripheral blood of volunteer donors. The cell suspension was further incubated without a stimulating agent and with PHA stimulation using mitogen (30 μg/ml concentration), as well as with the addition of the studied nanoparticles (30 μg/ml concentration). Finally, the concentration of cytokines was determined by means of an immunosorbent assay.
Results. Compared to spontaneous production, the stimulation of mononuclear cells with nanoparticles in the according concentration of nanoTiO2 resulted in
the production of the IL-1 increasing to 60.38±9.04 pkg/ml (1.79 times P<0.01), for nanoTiO2-Ag – 91.75±11.49 pkg/ml (2.73 times P<0.01), for nanoAg – 77.35±6.2 pkg/ml (2.3 times P<0.01).
When nanoparticles were added to the mononuclear cells in the according concentration of nanoTiO2-Ag, the production of IL-6 increased to 75.8 (CI 71.8-87.4) pkg/ml (5.41 times P<0.01), nanoAg – 52,0 (CI 46.0-63.1) pkg/ml (3.71 times P<0.05), compared to spontaneous production.
When mononuclear cells were stimulated with nanoparticles in the according concentration of nano-TiO2-Ag, the production of TNF-α increased to 80.3 (CI 68.0-82.4) pkg/ml (5.43 times P<0.01), nanoAg – 37,8 (CI 36.7-54.2) pkg/ml (2.55 times P<0.05), compared to spontaneous production.
Compared to spontaneous production, the stimulation of mononuclear cells with nanoparticles in the according concentration of nanoTiO2-Ag resulted in the
production of IL-4 increasing to 60.8 (CI 52.8-77.6) pkg/ml (3.45 times P<0.01), nanoAg - 41,7 (CI 34.7-49.1) pkg/ml (2.37 times P<0.01).
Conclusion. The in vitro experiments have evidenced that nanoparticles at a concentration of 30 μg/ml for nanoTiO2-Ag and nanoAg are central in increasing
functional activity of PMBC producing proinflammatory cytokines IL-1, IL-6, TNF-α and producing IL-4 in donors (P<0.05). The aforementioned data demonstrates a negative effect of nanoparticles on nanotechnology industry workers causing them to develop chronic inflammatory response and allergic reactions.

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Published
2021-11-28
How to Cite
Riabovol, V., Kurchenko, A., Yavorovskyi, A., Savchenko, V., & Taran, N. (2021). A STUDY OF THE INFLUENCE OF PHOTOACTIVE TITANIUM COMPOSITE NANOPARTICLES ON THE FUNCTIONAL ACTIVITY OF CYTOKINE-PRODUCING MONONUCLEAR BLOOD CELLS IN VITRO. Immunology and Allergy: Science and Practice, (3), 23-30. https://doi.org/10.37321/immunology.2021.3-03